Project #13657 - Summary

 

 

You must include your name, ID #, and title.  In general the following need to be answered:

1)    It must be typed and double spaced, no longer than 1 page.

2)    State the hypothesis that was tested.

3)    State how the hypothesis is relevant to the general topic that was investigated.

4)    State the methods in 1-2 sentences used to test the hypothesis. Do not copy the protocol in part or entirety from the laboratory manual, or point penalties will apply.

5)    State the results or trends seen in 1-2 sentences and whether they support the hypothesis tested.

Here is a sample summary:

Sample Summary

 

Protein Quantification of Cellular Fractions

 

            Cauliflower inflorescence tissue was fractionated by a cellular fractionation procedure.  The fractions were nuclear, mitochondrial, and cytosolic, also a crude homogenate fraction was saved.  The purpose of this experiment was to determine the concentration of protein in each of the samples.  I predict that the crude homogenate sample will have the highest concentration since it contains all parts of the cell.

            It is important to determine the concentration of the proteins in the various samples so that we can use the appropriate amount of protein in later experiments.  To determine protein levels a standard curve was constructed using known concentrations of the protein BSA.  Coomassie Blue which binds proteins and provides an absorbance that is easily quantifiable at 595 nm was used.  The 4 samples were then diluted, coomassie blue was added and the absorbance read.  By using the standard curve we were able to determine the level of protein in the diluted sample and therefore in the original samples.

            When the protein levels were compared I found that the crude homogenate had the highest concentration of proteins, which is what my hypothesis predicted.  Additionally the levels of proteins in the other fractions from highest to lowest were nuclear, cytosolic and mitochondrial.  This may suggest that there are a lot more proteins synthesized in the cytosol than the mitochondria.

 

Subject Science
Due By (Pacific Time) 09/30/2013 02:00 am
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