Project #15546 - In Falvella et.al. [1] the expression of the CHRNA5 gene is investigated.

 

I need the answers for the fooloing questions! by reading the attached article!

Please i need someone with knowledge of Biology!!!!

Thank you!

 

      a)Illustrate how you would place/design primers specific for each isoform. You don’t need to put in the specific nucleotide sequences but rather sketch where the primers should be located. These should be able to amplify a product from total RNA in a PCR reaction, so what specifications should your primers have?

 

b)     b)How can a polymorphism/SNP affect the level of gene expression?

Why can a SNP increase the risk of lung cancer?

 

c)      c)What could be the role/effect in the cell of the non-functional isoforms of CHRNA5?

 

d)     d) What part of the protein are the isoforms missing and what effect would this have on protein function?

 

e)      e) How is an antibody produced?

Include a description of an epitope.

  f)What is the difference between the surface and non-surface fraction (fig 5)?

 

Subject Science
Due By (Pacific Time) 10/30/2013 11:00 am
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