Project #18727 - genetic

.  The DNA of the plasmid pHUB1 is circular, double-stranded, and contains 5.7 kbp.  This plasmid carries a gene whose protein product confers resistance to tetracycline.  The DNA has one site each for the following restriction enzymes:  EcoRI, HpaI, BamHI, PstI, SalI, and BglII.  Cloning into the BamHI and SalI sites abolishes tetracycline resistance; cloning into the other sites does not.  Digestion with various restriction enzymes and combinations of enzymes yields DNA fragments whose sizes in kbp are shown below:

 

BamHI and SalI          0.7 and 5.0

BamHI and BglII       1.6 and 4.1

BamHI and EcoRI      2.6 and 3.1

SalI and EcoRI           2.4 and 3.3

SalI and BglII             2.3 and 3.4

SalI and PstI               2.7 and 3.0

PstI and EcoRI           0.3 and 5.4

PstI and BglII             0.7 and 5.0

SalI and HpaI             1.4 and 4.3

HpaI and PstI             1.6 and 4.1

 

 

Position the cleavage sites for all these enzymes on a map of the pHUB1 DNA.  Draw the map as a circle with 5.7 kbp marked off on the circumference.

 

 

Subject Science
Due By (Pacific Time) 12/04/2013 12:00 am
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