Project #29262 - writing a summery

Please listen to this link and write one to two pages summery.

https://www.dropbox.com/s/xmxwe2kbeczj1nm/Seminar10.m4a

Here some information you might need.

Dr. Mark Maupin, Assistant Professor at the Colorado School of Mines

Free radical kinetics in fuel biodegradation: from homology modeling to a detailed kinetic analysis


The fumarate addition reaction, catalyzed by the enzyme benzylsuccinate synthase (BSS), is considered to be one of the most intriguing and energetically challenging reactions in biology. BSS belongs to the glycyl radical enzyme family and catalyzes the fumarate addition reaction, which enables microorganisms to utilize hydrocarbons as an energy source under anaerobic conditions. Unfortunately, the extreme sensitivity of the glycyl radical to oxygen has hampered the structural and kinetic characterization of BSS, thereby limiting our knowledge on this enzyme. To enhance our molecular-level understanding of BSS, a computational approach involving homology modeling, docking studies, molecular dynamics (MD) simulations, and ab initio calculations has been used to deduce the structure of BSS’s catalytic subunit (BSSα), illuminate the molecular basis for the fumarate addition reaction, and determine the energetics and kinetics of the reaction mechanism. We have identified two conserved and distinct binding pockets at the BSSα active site: a hydrophobic pocket for toluene binding and a polar pocket for fumaric acid binding. The orientation of substrates at the active site observed in MD simulations is consistent with experimental observations of the syn addition of toluene to fumaric acid while the energetic and kinetic analysis has revealed that the H-abstraction reaction is the rate-determining step and the addition reaction appears to be nearly barrierless.


Bio: Mark Maupin is an assistant professor in the Department of Chemical and Biological Engineering at the Colorado School of Mines. He received his B.S. in Chemistry in 2000 and M.S. in Interdisciplinary Studies: Biochemistry in 2002 from Boise State University. Inspired by his M.S. work on the kinetics of horse liver alcohol dehydrogenase, he joined the University of Utah as a Henry Erying Fellow and received his Ph.D. in Physical Chemistry in 2008 under the guidance of Gregory A. Voth. After conducting a post-doctoral research appointment he accepted an appointment at the Colorado School of Mines in 2010. He has co-authored 21 papers in peer-reviewed journals including 4 papers in JACS and 3 covers. Mark is a member of Sigma Xi, the senior editor of AIMS Molecular Science, the Computer and Information Sciences Section Chair in the AAAS Pacific Division, and a full council member in the AAAS Pacific Division. His research interests include the study of ligand-gated ion channels for the treatment of neurodegenerative disorders in addition to proton conducting fuel cells, ionic liquids, cellulase enzymes, and glycyl radical enzymes for alternative energy purposes.

Subject Science
Due By (Pacific Time) 04/30/2014 02:00 pm
Report DMCA
TutorRating
pallavi

Chat Now!

out of 1971 reviews
More..
amosmm

Chat Now!

out of 766 reviews
More..
PhyzKyd

Chat Now!

out of 1164 reviews
More..
rajdeep77

Chat Now!

out of 721 reviews
More..
sctys

Chat Now!

out of 1600 reviews
More..
sharadgreen

Chat Now!

out of 770 reviews
More..
topnotcher

Chat Now!

out of 766 reviews
More..
XXXIAO

Chat Now!

out of 680 reviews
More..
All Rights Reserved. Copyright by AceMyHW.com - Copyright Policy