Project #39741 - URGENT HELP in Biological Treatment

3. In the lab, the students are incubating E.coli (bacteria) in the flask at ambient temperature. After preparing the glucose-based nutrient, they inoculated E.coli into the nutrient in the flask. The following are the cell growth (as an optical density measured by spectrophotometer) over time. Use this equation for calculating the cell concentration. The cell concentration (g cells/L) = 0.5 * the Optical density at 600nm. Time (d) Optical Density at 600nm 0 0.02 0.5 0.03 1 0.2 1.5 2.1 2 5 2.5 7.1 3 8.6 3.5 9 a) Find out the specific growth rate using the above data using the graphic analysis (3 pt). b) Calculate the doubling time of E.coli from this experiment (2 pt). 4. A laboratory experiment is conducted by adding the phenol-degrading mixed culture to the 100 mL phenol-contaminated water in the 500 mL flask. The phenol-degrading mixed culture has the following conditions and kinetic parameters: The maximum specific growth rate: 0.16/h, Yx/s=0.4 (g cells/g phenol) Ks (Monod’s constant): 200 mg/L, So (initial phenol conc.): 2 mg/L How much initial cell concentration is required to have the phenol concentration of 1 mg/L after 5 h (4 pt)?

Subject Science
Due By (Pacific Time) 09/11/2014 02:00 pm
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