Project #89495 - Biology lab assignment, easy only 3 pages Should only cost 30 dollars maximum
18 Science Tutors Online
Final Applied Lab Project (1 credit Lab Component)
Addresses course outcomes 1-5:
apply the scientific method to scientific investigations
state a scientific hypothesis and design a basic experiment
conduct an experiment, make observations, and collect data
use knowledge of biological principles to correctly interpret qualitative and quantitative information
use critical analysis to draw conclusions
Look for the question, form a hypothesis; identify independent and dependent variable; aim to have 4 or 5 data points for your graph ; explain method to obtain results; conclusion – does data support hypothesis?
This is the culminating assessment in BIOL 102. It is designed to assess your ability to apply the principles of the scientific method. For this project, you will complete the activity below. Make sure to address all points (questions) associated with the activity.
The Effect of low pH on Enzyme Activity
Design an experiment in which you will test the effect of an acidic fluid on enzymatic activity. (Recall: enzymes are proteins.) To complete this project, it may be useful for you to review the Scientific Method Tutorial (found the Course Content section of the classroom under the Science Learning Center link) and the Scientific Method lab (Lab 1), so that you can better understand how to design an experiment. It may also be helpful for you to review your textbook and Lab 4 (Enzymes). As you review Lab 4, you will be reminded that there are several factors that impact enzymatic activity: pH, temperature, and amount of reagent. Feel free to refer to observations and information from Lab 4 as you complete the Final Applied Project (see the questions below). Or in other words, it is OK to use the same enzyme/subtrate/method as you did in lab 4 (but modify the treatment), or you can search on-line to find a different enzyme/subtrate/method for measuring enzyme activity.
As you design your experiment for this project, please remember that you are trying to examine how an acidic fluid will modify the outcome of an enzymatic reaction. To successfully complete this project, you will need to identify the question(s) being asked in your experiment and the hypothesis that you are testing. In your experimental design, you must clearly explain what you are doing. That means that you will need to identify the enzyme and the acid, as well as explain your experimental protocol (this information will help you to answer question 2). You must also thoroughly explain how the addition of the acidic fluid impacted the overall reaction process (this information will help you to answer question 4).
Hint: Keep in mind that the acid will change the environmental conditions of the experiment (for example, a low pH value could change the shape of the active site on the enzyme protein), without directly participating in the reaction.
You may need all or some of the following, depending on your experimental design:
Materials from your lab kit:
hydrogen peroxide solution (you can purchase this at a pharmacy if you have used up the bottle that came with the lab kit)
plastic beakers or cups
yeast (can be purchased at grocery store if you need more)
marker for labeling of beakers
You may choose to use additional materials (different acidic solutions and/or different organisms and/or differnet subtrate(s) if you chose to look at an enzyme other than catalase).
Write a paper that includes the following:
1. Title page: title, your name, course name, semester
2. Introduction: introduce your project, include needed background information, the question(s) that you are asking and a clear hypothesis for your experiment.
3. Design an experiment. Provide a detailed account of the materials and methods used to conduct the experiment. Also include the methods for data collection and analysis.
4. Conduct the experiment and record your results. What did you observe? Present your data in table and/or graph . Remember to include the unit of measure.
5. Use your knowledge about enzymes and acids to interpret and discuss your results. It may be necessary for you to refer to your textbook and/or use other information resources. What effect does the acidic treatement have on the enzyme activity? Looking back, how could you have improved your experiment?
6. What is your conclusion? Was your hypothesis supported?
7. Cite all reference sources used (including text book) and provide a reference section with citations in APA format
Submit your final applied lab project as an attached Word document in the assignment folder by the due date specified in the course schedule
Rubric Name: Final Applied Lab Project
Background information about enzymes in general and about specific enzyme used in project is clearly and accurately written. Questions and hypothesis are specific, relevant and clearly stated.
Background information about enzymes in general and about specific enzyme is somewhat unclear and/or inaccurate. Questions and hypothesis could be more specific, relevant and clearly stated.
Missing background information about enzymes in general or about specific enzyme used in project or questions and hypothesis.
Missing background information about enzymes in general and about specific enzyme used in project and questions/hypothesis.
Experiment is designed to directly test the hypothesis; description of experiment is detailed and well written and includes all materials and methods used.
Experiment is mostly designed to test the hypothesis and/or description of experiment is somewhat inaccurate and/or some information about materials and methods used is missing and/or minor problems with clarity an organization.
Experiment is barely designed to test the hypothesis and/or description of experiment is inaccurate and/or most of the materials and methods used are missing and/or major problems with clarity an organization.
Experiment is not designed to test hypothesis and/or description of experiment and materials and methods used are missing.
Results are clearly and accurately presented in a table and/or graph format.
Results are presented, but minor problems with clarity and/or accuracy.
Results are described, but major problems with clarity and/or accuracy and/or results not presented in table or graph.
Results are not included, or major problems with clarity, accuracy or organization
A clear, accurate and well organized discussion of results that demonstrates good knowledge of enzymes.
Minor problems with clarity, accuracy or organization and/or demonstrates some gaps in knowledge of enzymes.
Major problems with clarity, accuracy or organization or demonstrates minimal knowledge of enzymes.
Missing discussion of results, or major problems with clarity, accuracy or organization and missing understanding of enzymes.
A well stated conclusion that is accurate and specific and directly related to the data collected. Clearly stated whether or not the results support the hypothesis. Relevant suggestion for improvement of experiment.
Conclusion stated, but slightly inaccurate, and/or not directly related to data collected and/or suggestion for improvement of experiment not very relevant.
Conclusion stated, but inaccurate and/or barely related to data collected and/or missing suggestion for improvement of experiment.
Missing conclusion, only suggestion for improvement of experiment.
All references used are included and in correct APA format, in-text references are included.
All references are included, but a few minor mistakes in APA format, and/or missing in-text references.
Missing one or several references and/or major mistakes in APA format.
No references included or references not in APA format.
0-3 minor spelling and grammatical errors
Between 4 and 7 minor spelling and grammatical errors